Efficiency of Different Extraction Solvents on Recovery of Histamine from Fresh, Frozen and Canned Fish

نویسندگان

  • A. Fazlara Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • F. Zolfaghar Karahroodi Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • H. Najafzadeh Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  • M. Zarei Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده مقاله:

Background: Histamine is a food-borne chemical hazard which causes scombroid poisoning. The efficiency of histamine recovery from fish is greatly influenced by selection of an appropriate extraction solvent. Hence, in this study the efficiency of different extraction solvents on recovery of histamine from fresh, frozen and canned fish was evaluated. Methods: Fresh, frozen and canned rainbow trout samples were homogenized in six different extraction solvents including 0.1 N hydrochloric acid, 5% trichloroacetic acid, 75% methanol, 75% ethanol, 75% methanol-0.4 N hydrochloric acid and 75% ethanol-0.4 N hydrochloric acid. Samples were evaluated for recovery of known quantities of histamine added to fish tissue prior to extraction. The percent values of the recoveries were compared using ANOVA (SPSS 16.0). Results: Findings of this experiment indicated that different extraction solvents provided different overall mean recoveries for histamine in fish tissue. Using a combination of acid and organic solvents provided a more efficient extraction solvent for histamine from fish tissue than acid or organic solvents alone. In addition, among the solvents used in this study, 75% ethanol-0.4 N HCl resulted in a more complete recovery of added histamine from fresh, frozen and canned fish tissue. Conclusion: In order to obtain a more accurate measure of histamine in fish tissue, 75% ethanol-0.4 N hydrochloric acid appears to be a good choice for extraction.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

efficiency of different extraction solvents on recovery of histamine from fresh, frozen and canned fish

background: histamine is a food-borne chemical hazard which causes scombroid poisoning. the efficiency of histamine recovery from fish is greatly influenced by selection of an appropriate extraction solvent. hence, in this study the efficiency of different extraction solvents on recovery of histamine from fresh, frozen and canned fish was evaluated. methods: fresh, frozen and canned rainbow tro...

متن کامل

extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties

آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...

15 صفحه اول

Comparison of carotenoid content in fresh, frozen and canned corn

The carotenoid content of fresh, commercially canned and frozen samples of two cultivars, White Shoepeg (WS) and Golden Whole Kernel (GWK) corn (Zea mays), from the same production field was studied. WS and GWK corn samples were harvested daily over a five-day-period and randomly selected for analysis as fresh, frozen or canned. Major carotenoids detected were lutein and zeaxanthin, and to a le...

متن کامل

Nutritional differences between canned, frozen, and fresh veggies?

Fresh, frozen, and canned vegetables all have their own unique set of benefits and drawbacks, but all contain vital nutrients, such as carbohydrates, protein, vitamins, minerals, and fiber, which are maintained through the respective processes. Canned and frozen versions are the most common processed vegetables. While processed vegetables can be more convenient, have a longer shelf-life, and ar...

متن کامل

Nutritional differences between canned, frozen, and fresh veggies?

Fresh, frozen, and canned vegetables all have their own unique set of benefits and drawbacks, but all contain vital nutrients, such as carbohydrates, protein, vitamins, minerals, and fiber, which are maintained through the respective processes. Canned and frozen versions are the most common processed vegetables. While processed vegetables can be more convenient, have a longer shelf-life, and ar...

متن کامل

Nutritional differences between canned, frozen, and fresh veggies?

Fresh, frozen, and canned vegetables all have their own unique set of benefits and drawbacks, but all contain vital nutrients, such as carbohydrates, protein, vitamins, minerals, and fiber, which are maintained through the respective processes. Canned and frozen versions are the most common processed vegetables. While processed vegetables can be more convenient, have a longer shelf-life, and ar...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 1  شماره 3

صفحات  72- 76

تاریخ انتشار 2014-09

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023